Invitro Evaluation of Antifungal Property of Ethanolic extract of Rosa Indica

Abstract

Introduction: Rose is a woody perennial flowering plant of Genus Rosa, within the family Rosaceae. Roses have a variety of health benefits.It acts as an antiseptic, antispasmodic, astringent, bactericidal (against typhoid, diarrhea, cholera, food poisoning), antiviral (cold or influenza). 80% of vital fatty acids and antioxidants are found in rose extract, which aids in skin cell regeneration. Rose extract is used to treat fungal infections, mainly candidiasis. Various studies have been conducted to establish the anti-fungal, anti-bacterial and anti-oxidant activities of rose. These activities were attributed to the presence of flavonoid and phenolic compounds, also known as bioactive agents Phytochemical analysis showed the presence of flavonoids and tannin alkaloids and carbohydrates were found in R. indica Rosa indica extract found antibacterial activity against Proteus sp. and E. coli, and antifungal activity against A. fumigatus strains. Aim & Objective: To Elucidate the antifungal efficacy of rose petals ( Rosa indica ) on Aspergillus niger , Candida albicans, Penicillium spp. Materials and Methods: 10 gm of fresh flower sample was weighed and soaked in 100 ml of ethanol. The extract was allowed to stand overnight and filtered using sterile filter paper. The antifungal activity of the sample was determined by well diffusion method on Potato Dextrose agar (PDA) medium. Then the microbial growth was determined by measuring the diameter of the zone of inhibition. Ketoconazole is used as a control, the outcomes were then contrasted with a control against Candida albicans, Aspergillus niger, and Penicillium species. Results: The zone of inhibition of rose extract was found to be 12mm against Aspergillus niger, 10mm and 13mm against Candida albicans and 13mm, 16mm, 20mm and 23mm against Penicilliumspp. This study shows the presence of antifungal activity in rose extract. Discussion: Rose petal extract possesses antifungal properties. Rose extract's zone of inhibition is less than that of the antifungal medication ketoconazole. The antifungal activity of rose extract is more efficient against Penicillium spp. when compared to Aspergillus niger and Candida albicans. Conclusion: It is true that rose have antifungal properties. Rose extract is more efficient against Penicillium spp in the current investigation. This discovery makes rose a powerful antifungal that can be used to treat fungal infections.

Keywords: Antifungal Property, Ethanolic extract, Rosa Indica