Staphylococcus aureus biofilm formation from the isolates of clinical cases: A prospective study

Abstract

Background: The aim of the present study is to assess the biofilm production among clinical isolates of Methicillin-Resistant Staphylococcus aureus (MRSA) in Tertiary Care Hospital, Central India. This aim were achieved under the following objectives such as antibiotic susceptibility pattern of Methicillin-Sensitive Staphylococcus aureus (MSSA) and MRSA among isolate and phenotypic pattern of MRSA and detection and prevalence of biofilm producing MSSA and MRSA isolated obtain from various clinical samples. Methods: This is a study that focuses on description and observation. Following receipt of approval on an ethical level from the Institutional Ethical Committee of Index Medical College Hospital & Research Centre in Indore, the research project was carried out.    Results: We observed highest number of pus isolates that are Biofilm producers in MSSA & MRSA, which accounts to 20 & 24 respectively. The strong Biofilm producers accounts to 7 cases in MSSA and 6 cases in MRSA cases out of 70 and 44 respectively. Whereas weak Biofilm producers accounts to 38 cases in MSSA and 28 cases in MRSA subjects. When compared between the two groups to identify the significance of Biofilm producing capacity, we observed a significant change (p <0.05).  Conclusion: Biofilm formation detection is a simple and cost-effective laboratory test that can be performed on a regular basis. A better understanding of biofilm will allow clinicians to better treat the infections, resulting in a lower mortality and morbidity rate for patients.