A DIAGNOSIS OF DENGUE BY NS1 ANTIGEN, ELISA AND REVERSE TRANSCRIPTASE PCR

Abstract

Background: Dengue fever is the further most common developing tropical viral illness affecting humans today. Dengue is a mosquito-borne disease spread largely by Aedes aegypti, however, Aedes albopictus has also been identified as a vector. Dengue NS1, lgM, and lgG ELISA assays are commonly used in ordinary laboratories to diagnose dengue infection. NS1 canister is found from the first to the seventh day after the start of symptoms, while anti-DENV lgM and lgG antibodies take 4-5 and 1-14 days, respectively, depending on whether the patient has a primary or secondary infection. Aim: the study is to assess role of NS1 antigen determination in diagnosis of dengue. Materials and Method:  ICT (Immunochromatography Test) ExDXTM Dengue Combo (Ag+Ab) is a rapid qualitative immune chromatographic test for Dengue detection of NS1antigen and variance detection of IgM and IgG antibodies against dengue virus in human serum. Result All 130 serum samples were tested for NS1 antigen using NS1 antigen ELISA kit and 63 (48.46%) were found to be positive and 67 (51.53%) for NS1 antigen. The NS1 antigen was detected from day 1 to day 7 of illness with maximum positivity 73.33 % on day 4 of illness.  Conclusion:  NS1 antigen detection and dengue group specific reverse transcriptase RT-PCR are valuable techniques for the rapid and early microbiological diagnosis of dengue.