Assessment of Buffalo Semen Preservability Using Tris Extender Enriched With Turmeric Extract
##plugins.themes.academic_pro.article.main##
Abstract
Abstract
Background and Objective: The freeze-thaw process results in structural and functional damages caused by over-accumulation of reactive oxygen species (ROS). Addition of exogenous antioxidants to semen extender is of a great importance to overcome the oxidative damage during the freezing process antioxidants to semen extender is of a great importance to overcome the oxidative damage during the freezing process. The objective was to evaluate the effect of tris citric acid fructose egg yolk (TCFY) extender supplemented with turmeric extract
Materials and Methods:Five tubes (each contains 5ml TCFY).The first tube contains no turmeric extract and kept as a control. The other four tubes contain turmeric extract as follows (100µl /5ml, 200 µl /5ml, 300 µl /5ml and 400 µl /5ml, v/v). Pooled bull semen were extended with Tris extender ( Tris with zero turmeric extract was kept as a control) . Turmeric extract was added to the tris extender at concentrations (100µl /5ml TT1, 200 µl /5ml TT2, 300 µl /5ml TT3 and 400 µl /5ml, TT4 v/v).. The semen samples was added and final sperm concentration 60 × 106/ml was attained. Extended semen were subjected to semen freezing protocol. Semen assessments including motility, alive%, abnormality%, intact sperm membrane (hypo-osmotic swelling test), and acrosome status were carried out for both cooled and frozen semen. Conception rate was carried out with the post-thawed semen.
Results: The post-cooling semen characteristics revealed improved sperm motility inTT1 and TT2, Sperm membrane integrity (HOST) significantly (P<0.016) ameliorated inTT3 and acrosome integrity significantly ((P<0.037) enhanced in TT1 as compared to the control. The post-thawing results exhibited significant (P<0.000) improvement in sperm motility in TT1 and TT2 and significant (P<0.004) amelioration in Sperm membrane integrity (HOST) inTT1, TT3and TT4 if compared to the control. The conception rate was the best in concentrations TT1 and TT2 if compared to the control and other concentrations.
Conclusion: It is concluded that, in cooled and post-thawed semen, the superior semen quality and Conception rate was attained in TT1 and TT2.
Keywords: Buffalo; Turmeric Extract; semen; Tris; cryopreservation.