Assessment of Bull Semen Cryopreservation Extended in Tris Extender Enriched with Green Tea Extract. Effect of green tea on cattle semen Section Original Research Paper

##plugins.themes.academic_pro.article.sidebar##

Published May 15, 2021
Download
PDF
Statistic

Downloads

Download data is not yet available.
Vol. 4 No. 03 (2021): International Journal of Medical Science in Clinical Research and Review

##plugins.themes.academic_pro.article.main##

Reda Ibrahim ELSheshtawy
Department of Animal Reproduction and Artificial Insemination, National Research Centre, Dokki, Giza, Egypt
Gamal Attia ELsisy
Walid said El-Nattat

Abstract

Background and Objective : Green tea has potential antioxidant capacity and free radical-scavenging properties. The present study aimed to clarify the effect of the watery green tea extract in tris semen extender on cryopreservability. Materials and Methods: 0.0, 0.2, 0.4, 0.6, 0.8, 1.0 and 1.2% of green tea extract were added to the Tris citrate egg-yolk fructose (TCYF) diluent. Semen was added to the different concentration of diluents to obtain 60 ×106 motile sperm.ml-1. Cooling and equilibration at 5°C proceeded and ended with freezing in liquid nitrogen. Thawed semen was evaluated for total sperm motility, viability, abnormality, membrane integrity (HOS) and normal intact acrosome%. Results: concerning chilled diluted semen, high motile and alive sperms % were found on addition of 0.2 and 0.4% green tea extract to the tris-basic extender, while all treatments with green tea extract exerted low abnormal sperm%. On the other hand, 0.6 and 0.8 % green tea extract addition showed significant increase in the membrane integrity and acrosome integrity% compared to the control. Concerning the thawed diluted semen, the addition of 0.4, 0.6, 0.8% green tea extract had maintained the motility % as that of the control,  increased live sperm%, lowered the abnormal sperm% and had no significant effect on membrane or acrosome integrities% compared to the control (P≤0.0001). Conclusion: green tea extract supplementation in concentration of 0.2, 0.4, 0.6, 0.8% to cattle bull extender protected sperm from the hazard effect of cryodamage and offer better protection for semen quality parameters.

##plugins.themes.academic_pro.article.details##

How to Cite
ELSheshtawy, R. I., ELsisy, G. A., & El-Nattat, W. said. (2021). Assessment of Bull Semen Cryopreservation Extended in Tris Extender Enriched with Green Tea Extract.: Effect of green tea on cattle semen. International Journal of Medical Science in Clinical Research and Review, 4(03), Page: 13–20. Retrieved from http://ijmscrr.in/index.php/ijmscrr/article/view/148

References

    . Agarwal, A. , Nallella, K.P., Allamaneni, S.S. & Said, T.M . (2004). Role of antioxidants in treatment of male infertility: an overview of the literature. Reprod Biomed online 8 (6): 616–627.
    2. Seddiki, Y., da Silva H.M. & da Silva, F.M. (2017). Antioxidant properties of polyphenols
    and their potential use in improvement of male fertility: A review. Biomed J Sci Tech Res 1(3) : 612-617doi: 10.26717/BJSTR.2017.01.000259.
    3. Mata-Campuzano, M. , Alvarez-Rodriguez M., Tamayo-Canul J., Lopez- Uruena E., de Paz, P., Anel, L. Martinez-Pastor , F. & Alvarez, M. (2014) . Refrigerated storage of ram sperm in presence of Trolox and GSH antioxidants: effect of temperature, extender and storage time. Anim Reprod Sci 151(3-4) :137-147. doi : 10.1016/j.anireprosci.2014.10.006
    4. Bucak, M. Ataman M.B., N., S Baspinar N., Uysal O., , Taspinar M.,. Bilgili, A, Ozturk C., Gungor, S. Inanc M.E. & Akal E. (2015) . Lycopene and. resveratrol improve post-thaw bull sperm parameters: sperm motility, mitochondrial activity and DNA integrity. Andrologia, 47(5) : 545-552. doi: 10.1111/and.12301. Epub 2014 Jun 9.

    5. Sariozkan, S. , Tuncer P.B., Buyukleblebici S., Bucak M.N.F.& Eken Canturk, A. (2015) .Antioxidative effects of cysteamine, hyaluronan and fetuin on post-thaw semen quality, DNA integrity and oxidative stress parameters in the brown swiss bull. Andrologia, 47(2): 138-147. doi : 10.1111/and.12236.
    6. Sreejith, N., Brar, A.S., Ahuja C.S.,. Sangha P.S, Chaudhary K.C. (2006) . A comparative study on lipid peroxidation, activities of antioxidant enzymes and viability of cattle and buffalo bull spermatozoa during storage at refrigeration temperature. Anim Reprod Sci, 96(1-2) : 21-29. https://doi.org/10.1016/j.anireprosci.2005.11.002.
    7. Nichi, N.,. Bols P.E.J, Züge R.M., Barnabe V.H., Goovaerts I.G.F., Barnabe R.C. & Cortada C.N.M. (2006) . Seasonal variation in semen quality in Bos indicus and Bos taurus bulls raised under tropical conditions. Theriogenology, 66(4) : 822-828. https://doi.org/10.1016/j.theriogenology.2006.01.056.
    8. Bilodeau, J.F., Chatterjee S., Sirard M.A. & Gagnon C. (2000) . Levels of antioxidant defenses are decreased in bovine spermatozoa after a cycle of freezing and thawing. Mol Reprod. Dev. ,55(3) :282-288. doi: 10.1002/(SICI)1098-2795(200003)55:3<282::AID-MRD6>3.0.CO;2-7.
    9. Anghel, A., Zamfirescu, S., Dragomir, C., Nadolu, D., Elena, S. & Florica, B. (2010) .The effects of antioxidants on the cytological parameters of cryopreserved buck semen. Rom Biotech Lett , 15(3) :26-32.
    10. Ansari, M.S., Rakha, B.A., Andrabi, S.M.H., Ullah, N., Iqbal, R., Holt, W.V. & Akhter S.(2012).Glutathione-supplemented tris-citric acid extender improves the post-thaw quality and in vivo fertility of buffalo (Bubalus bubalis) bull spermatozoa. Reprod Biol , 12(3) :271-276. doi : 10.1016/j.repbio.2012.10.001.
    11. Beconi, M.T., Francia, C.R., Mora, N.G. & Affranchino, M.A. (1993) . Effect of natural antioxidants on frozen bovine semen preservation. Theriogenology; 40 (4) : 841–51. https://doi.org/10.1016/0093-691X(93)90219-U.
    12. Avdatek F., Yeni D., İnanç M.E., Çil B., Tuncer P.B., Türkmenand R. & Taşdemir U. (2018) .Supplementation of quercetin for advanced DNA integrity in bull semen cryopreservation. Andrologia, 50 :. doi: 10.1111/and.12975.
    13. Moretti, E., Mazzi, L., Bonech, C., Salvatici, M.C., Iacoponi, F., Rossi C., Collodel G. (2016). Effect of quercetin-loaded liposomes on induced oxidative stress in human spermatozoa. Reprod Toxicol , 60: 140-147. doi : 10.1016/j.reprotox.2016.02.012.
    14. Frei, B.& Higdon, J.V. (2003). Antioxidant activity of tea polyphenols in vivo: evidence from animal studies. J. Nutr., 133(10) , 3275S-384S. https://doi.org/10.1093/jn/133.10.3275S.
    15. Nagle, C.M., Olsen, C.M , Bain, C.J.& Whiteman, A. (2010) .Green and PM Webb. Tea consumption and risk of ovarian cancer. Cancer Causes Control, 21(9) : 1485–1491. doi : 10.1007/s10552-010-9577-7.
    16. Forester, S.C. and J.D. Lambert, 2011. Antioxidant effects of green tea. Mol. Nutr. Food Res., 55(6) : 844–854. doi: 10.1002/mnfr.201000641.
    17. Wittayarat, M., Ito, A., Kimura, T., Namula, Z., Luu, V.V., Do, L.T., Sato, Y., Taniguchi, M. & Otoi, T. (2013) . Effects of green tea polyphenol on the quality of canine semen after long-term storage at 5°C. Reprod Biol 13(3) : 251-254. doi: 10.1016/j.repbio.2013.07.006.
    18. Zanchia, M.M., Manfredinib, V., dos, D., Bruma, S., Vargasa, L.M., Spiazzia, C.C., Soaresa, M.B., Izaguirrya, A.P. & Santosa, F.W. (2015). Green tea infusion improves cyclophosphamide-induced damage on male mice reproductive system. Toxicol. Reports; 2: 252–260. https://doi.org/10.1016/j.toxrep.2014.12.016.
    19. Ali, H., Riaz, A., Ghafoor, A., Javeed, A., Ashraf, M. & Satter, A. (2014) . Antioxidative protection by Strawberry and green tea extracts during cryopreservation of Sahiwal bull semen. Pak. J. Life. Soc. Sci., 12(2) : 97-100.
    20. Mehdipour, M., Kia, H.D., Najafi, A., Dodaran, H.V. & García-Álvarez, O. (2016). Effect of green tea (Camellia sinensis) extract and pre-freezing equilibration time on the post-thawing quality of ram semen cryopreserved in a soybean lecithin-based extender. Cryobiology, 73(3) : 297-303. doi: 10.1016/j.cryobiol.2016.10.008. Epub 2016 Oct 28.
    21. Inanç, M.E., Çil, B., Yeni, D., Avdatek, F., Orakçi, D., Tuncer, P.B., Türkmen, R. & Taşdemir, U. (2019) .The Effect of Green Tea Extract Supplementation in Bull Semen Cryopreservation. Kafkas Univ Vet Fak , 25(5) : 703-708. doi : 10.9775/kvfd.2019.21702.
    22. Ahmed, H., Jahan, S., Khan, A., Khan, L., Khan, B.T., Ullah, H., Riaz , M. & Ullah , K. (2020). Supplementation of green tea extract (GTE) in extender improves structural and functional characteristics, total antioxidant capacity and in vivo fertility of buffalo (Bubalus bubalis) bull spermatozoa. Theriogenology, 145:190-197. doi: 10.1016/j.theriogenology.2019.10.024.
    23. Foote, R.H. (1970) . Fertility of bull semen at high extension rates in Tris buffered extenders. J Dairy Sci., 53(10) :1475-1477. https://doi.org/10.3168/jds.S0022-0302(70)86417-7.
    24. Graham, E.F., Schmehl, M.K.L. & Maki-Laurila, M. (1970) . Some physical and chemical methods of evaluating semen. Proc. 3rd NAAB Tech. Conf. Artif. Insemin. Reprod., 12–14 April Milwaukee, WI, National Association of Animal Breeders, Columbia, MO pp. 44-48.
    25. Agarwal, A. , Gupta, S. & Sharma, R. (2016) . Andrological evaluation of male infertility: A laboratory guide. Springer International Publishing Switzerland ; p. 80.
    26. Revell, S.G. & Mrode, R.A. (1994) . An osmotic resistance test for bovine semen. Anim Reprod Sci , 36: 77-86.
    27. Watson, P.F. (1975) . Use of giemsa stain to detect changes in the acrosome of frozen ram spermatozoa. Vet. Record, 97(1) : 12-15. doi: 10.1136/vr.97.1.12.
    28. Snedecor, G. W. & Cochran, W. G. (1989). Statistical methods, 7th Edn. Ames: Iowa State Univ. Press Iowa, 359-364.
    29.Chatterjee, S.& Gagnon C. (2001) . Production of reactive oxygen species by spermatozoa undergoing cooling, freezing, and thawing. Mol Reprod Dev , 59(4) : 451-458. doi: 10.1002/mrd.1052.
    30. Park, SH, Yu I.J. (2017) . Effect of Antioxidant Supplementation in Freezing Extender on Porcine Sperm Viability, Motility and Reactive Oxygen Species. J Emb Trans , 32(1) : 9-15.doi: G704-001405.2017.32.1.004.
    31. De Amicis, F., Santoro M., Guido C., Russo A. & Aquila S. (2012) . Epigallocatechin gallate affects survival and metabolism of human sperm. Mol Nutr Food Res , 56(11) :1655-1664. doi : 10.1002/mnfr.201200190.
    32. Lombardo, F., Fiducia M., Lunghi R., Marchetti L., Palumbo A., Rizzo F., Koverech A., Lenzi A. & Gandini L. (2012) . Effects of a dietary supplement on chronic pelvic pain syndrome (Category IIIA), leucocytospermia and semen parameters. Andrologia, 44(1) :672–678. doi: 10.1111/j.1439-0272.2011.01248.x.

    33. Khan, H. , Khan, M., Qureshi, M.S., Ahmad, S., Gohar, A., Ullah, H., Ullah, F., Hussain, A., Khatri, P. , Shah, S.S.A , Rehman , H. , & Khan , A. (2017) . Effect of green
    tea extract (Camellia sinensis) on fertility indicators of post-thawed bull
    spermatozoa. Pak J Zool , 49(4) :1243-1243. doi : 10.17582/journal.pjz/2017.49.4.1243.1249
    34. Najafi, A., Kia, H..D. , Mohammadi, H., Najafi, M.H., Zanganeh, Z., Sharafi, M., Martinez, P.F. & Adeldust, H. (2014) . Different concentrations of cysteamine and ergothioneine improve microscopic and oxidative parameters in ram semen frozen with a soybean lecithin extender. Cryobiology, 69(1) : 68-73. doi : 10.1016/j.cryobiol.2014.05.004.
    35. Sharafi, M., Zhandi M. & Sharif A.A. (2015) . Supplementation of soybean lecithin-based
    semen extender by antioxidants: complementary flowcytometric study on
    post-thawed ram spermatozoa. Cell Tissue Bank , 16(2) : 261-269. doi: 10.1007/s10561-014-9458-5.
    36. Gale, I., Gil, L. , Malo, C., Gonzalez, N. & Martinez, F. (2015) . Effect of Camellia sinensis supplementation and increasing holding time on quality of cryopreserved boar semen. Andrologia, 47(5) : 505–512. doi: 10.1111/and.12293.
    37. Roychoudhury, S., Agarwal, A., Virk, G. & Cho, C.L. (2017) . Potential role of green tea catechins in the management of oxidative stress-associated infertility. Reprod Biomed Online, 34(5) : 487 – 498. doi: 10.1016/j.rbmo.2017.02.006.
    38. Hashim, F., Tvrda, E., Mass anyi , P., Stawarz , R. & Lukac , N. (2016) . Effects of biological active substances to the spermatozoa quality. J Microbiol Biotechnol Food Sci , 5(3) : 263-267. doi: 10.15414/jmbfs.2015/16.5.3.263-267.
    39. Hijazi, M.M., Khatoon N., Azmi M.A., Rajput M.T., Zaidi S.I., Azmi M.A., Perveen R., Naqvi S.N. & Rashid M. (2015) .Report. effects of Camellia sinensis L. (green tea) extract on the body and testicular weight changes in adult Wistar rate. Pak J Pharm Sci , 28(1) : 249–253. Pubmed
    40. Hyon, S.H.A. (2004) . non-frozen living tissue bank for allotransplantation using green tea polyphenols. Yonsei Med J , 45(6) : 1025-1034doi: 10.3349/ymj.2004.45.6.1025.
    41. Dias, T.R., Alves M.G., Tomás G.D., Socorro S., Silva B.M. & Oliveira P.F. (2014) .White tea as a promising antioxidant medium additive for sperm storage at room temperature: A comparative study with green tea. J Agric Food Chem, 62(3) : 608-617. doi: 10.1021/jf4049462.
    42. Nouri, H. , Shojaeian K., Samadian F., Lee S., Kohram H., Lee J.I. (2018) . Using
    resveratrol and epigallocatechin-3-gallate to improve cryopreservation of stallion spermatozoa with low quality. J Equine Vet Sci , 70:18-25. https://doi.org/10.1016/j.jevs.2018.07.003.